|
KMID : 1007520080170030655
|
|
Food Science and Biotechnology
2008 Volume.17 No. 3 p.655 ~ p.658
|
|
|
Comparative Analysis of Tagatose Productivity of Immobilized LArabinose Isomerase Expressed in Escherichia coli and Bacillus subtilis
|
|
Cheon Ji-Na
Kim Seong-Bo
Park Seong-Won
Han Jong-Kwon
Kim Pil
|
|
|
Abstract
|
|
|
|
Although arabinose isomerase (E.C. 5.3.1.4), a commercial enzyme for edible tagatose bioconversion, can be expressed in an Escherichia coli system, this expression system might leave noxious by-products in food. To develop an eligible tagatose bioconversion with food-safe system, we compared the tagatose production activity of immobilized arabinose isomerase expressed in Bacillus subtilis (a host generally recognized as safe) with that of the enzyme expressed in E. coli. A 48% increase in tagatose production (4.3 g tagatose/L at 69.4 mg/L·hr) was found using the B. subtilis-expressed immobilized enzyme system, compared to the E. coli-expressed enzyme system (2.9 g tagatose/L). The increased productivity with safety of the B. subtilis-expressed arabinose isomerase suggests that it is a more eligible candidate for commercial tagatose production.
|
|
|
KEYWORD
|
|
|
tagatose bioconversion, food safety, expression host, Bacillus subtilis, Escerichia coli
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|